Killing a cell is a relatively straightforward affair, but selectively repairing damaged DNA is far more tricky.


CRG (Luis Serrano & Mark Isalan)

Becskei, A. & Serrano, L. (2000). Nature. Engineering stability in gene networks by autoregulation. Nature 405, 590-593.

Becskei, A., B. Seraphin, et al. (2001). "Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion." EMBO J 20(10): 2528-35.

Isalan, M., Klug, A. & Choo, Y. A rapid universal method for the design of customised zinc finger DNA-binding domains illustrated by targetting the HIV-1 promoter. Nature Biotechnology, Vol. 19 (7), 656-60 (2001).

Guerois, R., J. E. Nielsen, et al. (2002). "Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations." J Mol Biol 320(2): 369-87.

Mendes, J., R. Guerois, et al. (2002). "Energy estimation in protein design." Curr Opin Struct Biol 12(4): 441-6.

Reina, J., E. Lacroix, et al. (2002). "Computer-aided design of a PDZ domain to recognize new target sequences." Nat Struct Biol 9(8): 621-7.

Isalan, M., Lemerle C. & Serrano, L. Engineering gene networks to emulate Drosophila embryonic pattern formation. PLoS Biology 3 e64 (2005). [See also: Nature Rev Gen 6: 255 (2005) & Nature Methods 2: 246 (2005).]

Isalan, M., Santori, M. I., Gonzalez, C. & Serrano, L. Localized transfection on arrays of magnetic beads coated with PCR products. Nature Methods 2: 113-118 (2005).

CNIO (Amancio Carnero)

Hudson JD, Shoaibi MA, Maestro R, Carnero A, Hannon GJ, Beach DH. A proinflammatory cytokine inhibits p53 tumor suppressor activity. J Exp Med. 1999 Nov 15;190(10):1375-82.

Hannon GJ, Sun P, Carnero A, Xie LY, Maestro R, Conklin DS, Beach D. MaRX: an approach to genetics in mammalian cells. Science. 1999 Feb 19;283(5405):1129-30. No abstract available.

Carnero A, Hudson JD, Hannon GJ, Beach DH. Loss-of-function genetics in mammalian cells: the p53 tumor suppressor model. Nucleic Acids Res. 2000 Jun 1;28(11):2234-41.

Carnero A, Hudson JD, Price CM, Beach DH. p16INK4A and p19ARF act in overlapping pathways in cellular immortalization. Nat Cell Biol. 2000 Mar;2(3):148-55.

Maria E. Castro, Maria V. Guijarro, Victoria Moneo and A. Carnero; Cellular senescence induced by p53-ras cooperation is independent of p21waf1. In press. Journal of Cellular Biochemistry.

Cellectis (Frédéric Pâques)

Epinat JC, Arnould S et al.: A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells. Nucleic Acids Res. 2003 Jun 1;31(11):2952-62.

MHH (Florian Kuehnel)

Kuhnel F, Fritsch C, et al.: Doxycycline regulation in a single retroviral vector by an autoregulatory loop facilitates controlled gene expression in liver cells. Nucleic Acids Res. 2004 Feb 13;32(3):e30.

Kuhnel F, Zender L, et al.: Tumor-specific adenoviral gene therapy: transcriptional repression of gene expression by utilizing p53-signal transduction pathways. Cancer Gene Ther. 2004 Jan;11(1):28-40.

Zender L, Hutker S, Liedtke C, Tillmann HL, Zender S, Mundt B, Waltemathe M, Gosling T, Flemming P, Malek NP, Trautwein C, Manns MP, Kuhnel F, Kubicka S: Caspase 8 small interfering RNA prevents acute liver failure in mice. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7797-802.

Wirth T, Zender L, Schulte B, Mundt B, Plentz R, Rudolph KL, Manns MP, Kubicka S, Kuhnel F: A telomerase-dependent conditionally replicating adenovirus for selective treatment of cancer. Cancer Res. 2003 Jun 15;63(12):3181-8.

Kuhnel F, Zender L et al.: NFkappaB mediates apoptosis through transcriptional activation of Fas (CD95) in adenoviral hepatitis. J Biol Chem. 2000 Mar 3;275(9):6421-7.

In press:

EMBL groups receive EU funding to study protein complexes and gene networks

Two of the most important issues in biology right now are how the set of proteins produced by cells work together in complexes and how these molecular machines participate in pathways and networks. These questions are crucial to understanding both the systems that work in healthy and diseased cells.........

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